I still cant seem to get this to work, I have the Image I need of a leaf and am trying to make it transparent. After all of this is set up using a mix shader and transparent shader the image is clearly found by Blender. In the Material view-port you can see the image on the plane, However in Rendered view it comes up with the missing pink colour. Please try and just chuck ideas out of how to help. Any ideas will be tested. Thanks Guys
Usually when an object turns pink it is because the original file got moved since you last opened Blender.
It is still showing up in your 3D window because that is using a different part of ram.
I would relocate the file and load it again and make sure "leaf" (leave lol) it where it is once you bring it in to Blender.